Huvec Primary Cell Line
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Passage normal huvec cells when culture has reached approximately 80 confluence.
Huvec primary cell line. Primary human umbilical vein endothelial cells huvec are isolated from the vein of the umbilical cord and are commonly used for physiological and pharmacological investigations such as macromolecule transport blood coagulation angiogenesis and fibrinolysis. Many of the endothelial processes are commonly studied in vitro using human umbilical vein endothelial cells huvec or large vessel and microvascular endothelial cells isolated from various body tissues. Promocell offers huvec isolated in standard endothelial cell growth medium. All cells test negative for mycoplasma bacteria yeast and fungi.
Primary human umbilical vein endothelial cells huvec are isolated from human umbilical vein and cryopreserved at the end of the primary culture the cells are. Warm both the trypsin edta for primary cells atcc pcs 999 003 and the trypsin neutralizing solution atcc pcs 999 004 to room temperature prior to dissociation. Human umbilical vein endothelial cells huvec provide a classic model system to study many aspects of endothelial function and disease such as normal abnormal and tumor associated angiogenesis oxidative stress hypoxia and inflammation related pathways in endothelia under normal and pathological conditions cardiovascular related complications associated with various diseases mode of. Normal human umbilical vein endothelial cells huvec primary 309 09.
Human umbilical vein endothelial cells huvec 10 donor pool 233 08. Pipette 20 ml of endothelial cell growth medium to a t 75 flask. Lonza offers a comprehensive range of normal human primary endothelial cells and supporting media for cardiac aortic coronary iliac artery pulmonary artery cardiac microvascular. Take the endothelial cell growth medium from the refrigerator decontaminate the bottle with 70 alcohol in a sterile hood.
Human umbilical vein endothelial cells huvec 10 donor pool. Passage normal huvec cells when culture has reached approximately 80 confluence. Normal human umbilical vein endothelial cells huvec primary 309 09. Warm complete growth medium to 37 c prior to use with the cells.
Keep the medium to surface area ratio at 1ml per 5 cm 2. Performance testedat least 16 population doublings when cultured in medium 200 supplemented with low serum growth supplement lsgs guarant. Warm complete growth medium to 37 c prior to use with the cells. Warm both the trypsin edta for primary cells atcc pcs 999 003 and the trypsin neutralizing solution atcc pcs 999 004 to room temperature prior to dissociation.
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