Human Nanog Antibody Cell Signaling

Secondary all lanes.

Human nanog antibody cell signaling. Anti nanog antibody ab80892 at 1 500 dilution lane 1. Human nanog protein shares 52 overall amino acid identity with the mouse protein and 85 identity in the homeodomain. Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog oct 4 sox2 klf4 c myc and lin28 2 3. Nccit human pluripotent embryonic carcinoma epithelial cell whole cell lysates with 5 nfdm tbst lane 2.

Hela human cervix adenocarcinoma epithelial cell whole cell lysates with 5 nfdm tbst lane 3. Mouse embryonic stem cell line d3 lanes 4 5. Invitrogen anti nanog monoclonal hnanog 2 ebioscience catalog 14 5768 82. Nanog in adlf1 and fab2 stem cell lines.

Performed under reducing conditions. 1 1000 the hrp conjugated anti mouse igg antibody gtx213111 01 was used to detect the primary antibody. Anti nanog antibody epr2027 2 ab109250 at 1 2000 dilution lane 1. Nanog antibody gt3312 detects nanog protein by western blot analysis.

Goat anti rabbit hrp at 1 2000 dilution developed using the ecl technique. Chromatin immunoprecipitations were performed with cross linked chromatin from nccit cells and either nanog antibody or normal rabbit igg 2729 using simplechip enzymatic chromatin ip kit magnetic beads 9003. This antibody reacts with human samples. Human nanog maps to gene locus 12p13 31 while the mouse nanog maps to gene loci 6 f2.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human nanog protein. It is expressed in the central portion of the morula the inner cell mass of the blastocyst in embryonic stem cells esc and embryonic germ cells egc. Background nanog is a homeodomain containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells 1. Nanog mrna is present in pluripotent mouse and human cell lines and absent from differentiated cells.

Nanog was detected in immersion fixed adlf1 top panel and fab2 bottom panel induced pluripotent stem cell lines using goat anti human nanog antigen affinity purified polyclonal antibody catalog af1997 at 10 µg ml for 3 hours at room temperature. Nanog is thought to be a key factor in maintaining pluripotency. Tested in western blot wb immunofluorescence if immunocytochemistry icc and immunohistochemistry paraffin ihc p applications. Clone rea314 recognizes the human nanog antigen a homeobox transcription factor also known as es cell associated protein 4.

Mutant mouse embryonic stem cell line known to not express nanog lysates proteins at 200000 cells per lane. Nanog expression is controlled by a network of factors including sox2 and the key pluripotency regulator oct 4 1. The enriched dna was quantified by real time pcr using simplechip human oct 4 promoter primers 4641 simplechip human sox2 promoter primers 4649 and simplechip human α. Marker lanes 2 3.

Hek 293 human embryonic kidney epithelial cell whole cell lysates with 5 nfdm tbst.