Human Mammary Epithelial Cells Culture Medium

The promocell mammary epithelial cell growth medium is a serum free medium developed for the cultivation of epithelial cells from the mammary gland.

Human mammary epithelial cells culture medium. 3 0 culture of human mammary epithelial cells using epicult b or epicult c medium human human mammary epithelial cell cultures should be initiated from single cell suspensions refer to section 2 2. Humec medium is a serum free medium engineered specifically for the culture of human mammary epithelial cells hmec. Although all promocell media are optimized for use with primary human cells we have received feedback from customers that this particular medium can also be used for rat and murine mammary. Common uses of hmec include the study of breast cancer development three dimensional culture and carcinogen screening.

Mammary epithelial cells mepic lie within the alveolar lumen of the breast lobules which together with mammary ducts and adipose tissues form a complex network in the mammary gland. This translates to greater experimental validity more reproducible results and reduction of lot to lot variability. Basal cells are marked by the expression of cytokeratin 5 green and luminal cells are marked by the expression of cytokeratin 18 red. Enhanced growth of human mammary cells can be achieved by pre coating.

Otherwise cells will not adhere well to the tissue culture flask. Humec medium supports superior cell growth in the first culture after thaw and throughout the guaranteed lifespan when compared to a major competitors culture system figure 1. Mammary epithelial cell basal medium contains essential and non essential amino acids vitamins other organic compounds trace minerals and inorganic salts. Specialized airway and renal epithelial media support optimal growth of each cell type throughout numerous passages.

Mepic respond to various growth factors and hormonal cues and unde. Primary human epithelial cells isolated from lung nasal mucosa trachea bronchi and distal respiratory tract and renal tissue whole kidney and renal cortex. Mammary epithelial cell basal medium is a sterile phenol red free liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Human mammary epithelial cells in 2d culture.

Engineered specifically for culture of human mammary epithelial cells humec medium is a serum free formulation with frozen supplements containing growth factor and other components. Selected publications out of many where our customers have used megm growth media with various normal breast cells.